Tech p Tile.png

Simple and scalable genome analysis with TELL-Seq™

Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) is a simple and scalable NGS library technology to generate barcode linked-reads for genome scale haplotype phasing and/or de novo sequencing. In a PCR tube, under a standard NGS laboratory setting and without a need for any expensive protocol-specific instrument, TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit will generate an Illumina sequencing library in 3 hours. Multiple samples can be easily processed parallelly in a 96-well format when needed. The library protocol can be adjusted and used for various sizes of genomes ranging from bacteria to human. TELL-Seq™ library will replace both fragment library and mate-pair library altogether and become a new standard library method for WGS.


TELL-Seq™ Library Preparation and Sequencing Workflow


TELL-Seq Library Preparation and Sequencing Workflow

 

Methods and materials


Methods

  • Standard NGS laboratory setting without any protocol specific capital expenditure

  • All barcoding reactions are done in a 0.2ml PCR tube

  • 0.5ng – 5ng genomic DNA input based on the genome size

  • Works for both small and large genomes ranging from bacteria to human genome

  • Highly scalable workflow for preparation of multiple samples in parallel

 

Materials

TELL-Seq™ WGS Library Prep kit includes:

• TELL-Seq™ WGS Library Reagent Box 1

• TELL-Seq™ WGS Library Reagent Box 2

• TELL-Seq™ Library Index Primer Kit

• TELL-Seq™ Illumina Sequencing Primer KiT

 

Results


Results II: TELL-Seq Results for Three Bacteria with Different

Results I: IGV Snapshot of TELL-Seq Linked Reads

Results I: IGV Snapshot of TELL-Seq Linked Reads

Results II: TELL-Seq Results for Three Bacteria with Different

 

 

Results IV: De Novo Assembly Results Compared with reference

Results III: TELL-Seq Results Compared with Mate Pair Data

Results III: TELL-Seq Results Compared with Mate Pair Data

Novo Assembly Results Compared with Reference

 

 

Conclusion

Results V: Whole Genome Haplotype Phasing Results for NA12878

Results V: Whole Genome Haplotype Phasing Results for NA12878

TELL-Seq™ library technology enables short read 2nd generation sequencing systems to generate long read like sequencing data with innovative molecular barcoding library method. The technology offers an approach that is innovative and adaptable:

• Used for various sized genomes

• Single tube reaction without specialized instrument

• Highly scalable for doing multiple samples in parallel

• One-stop solution for de novo sequencing and whole genome phasing

• Automation Friendly